Isolation and Characterization of Bacterial Polyhydroxybutyrate Inclusions
نویسنده
چکیده
Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHI3 within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13c NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHI3 inclusions is proposed. Thesis Supervisor: ChoKyun Rha Title: Professor of Biomaterials Science and Engineering
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